01RXXPE

A.A. 2022/23

Course Language

Inglese

Course degree

Master of science-level of the Bologna process in Nanotechnologies For Icts (Nanotecnologie Per Le Ict) - Torino/Grenoble/Losanna

Course structure

Teaching | Hours |
---|---|

Lezioni | 40 |

Esercitazioni in laboratorio | 20 |

Teachers

Teacher | Status | SSD | h.Les | h.Ex | h.Lab | h.Tut | Years teaching |
---|---|---|---|---|---|---|---|

Di Fabrizio Enzo Mario | Professore Ordinario | FIS/07 | 40 | 0 | 20 | 0 | 3 |

Teaching assistant

Context

SSD | CFU | Activities | Area context |
---|---|---|---|

FIS/03 | 6 | F - Altre attività (art. 10) | Altre conoscenze utili per l'inserimento nel mondo del lavoro |

2022/23

This course aims to provide the mathematical and conceptual tools for understanding modern optics, in the framework of optical imaging systems. Specifically, the theoretical foundations of physical optics will be introduced, together with a number of applications relevant in the field of nano-bioscience. Starting from the classical description of electromagnetic wave propagation, the geometrical and physical optics approximations will be introduced, while discussing their applicability limits. Image formation is addressed for both scalar and fully vectorial electromagnetic fields, by mean of a Fourier Transformation formalism. The image formation mechanism is explained through the concepts of spatial resolution, point spread function and transfer function of optical elements, for both coherent and incoherent illumination. As application examples, the wide-field microscope and the scanning confocal microscope will be considered, wherein aberrations and image distortion (chromatic aberrations, vignetting, coma, spherical aberration, etc.) are treated mathematically. More advanced microscopy techniques based on the use fluorescent probes will be also described, such as STED and FLIM. In the second part of the course, the principles of temporal and spatial coherence of electromagnetic radiation will be addressed, with particular attention to the relationships between extended sources and spatial coherence (Van Cittert-Zernike theorem), spectral distribution and temporal coherence (Weiner-Khinchin theorem). Interferometric approaches to optical imaging will be introduced based on speckle analysis and wavefront analysis. Finally, specific applications of holographic and interferometric imaging will be explained (e.g. Mach-Zehnder microscopy, Quantitative Phase Imaging, Optical Coherence Tomography), by means of optoelectronic devices such as Wavefront Analysers and Spatial Light Modulators.

This course aims to provide the mathematical and conceptual tools for understanding modern optics, in the framework of optical imaging systems. Specifically, the theoretical foundations of physical optics will be introduced, together with a number of applications relevant in the field of nano-bioscience. Starting from the classical description of electromagnetic wave propagation, the geometrical and physical optics approximations will be introduced, while discussing their applicability limits. Image formation is addressed for both scalar and fully vectorial electromagnetic fields, by mean of a Fourier Transformation formalism. The image formation mechanism is explained through the concepts of spatial resolution, point spread function and transfer function of optical elements, for both coherent and incoherent illumination. As application examples, the wide-field microscope and the scanning confocal microscope will be considered, wherein aberrations and image distortion (chromatic aberrations, vignetting, coma, spherical aberration, etc.) are treated mathematically. More advanced microscopy techniques based on the use fluorescent probes will be also described. In the second part of the course, the principles of temporal and spatial coherence of electromagnetic radiation will be addressed, with particular attention to the relationships between extended sources and spatial coherence (Van Cittert-Zernike theorem), spectral distribution and temporal coherence (Weiner-Khinchin theorem). Interferometric approaches to optical imaging will be introduced based on speckle analysis and wavefront analysis.

The student is expected to acquire knowledge in:
- fundamental principles of optical imaging
- operation of most widely used microscopy/interferometric techniques with particular regard to the nano-bio domain Expected skills are:
- ability to understand the underlying mechanisms of complex imaging systems
- ability to design and operate optical systems for specific imaging purposes

The student is expected to acquire knowledge in:
- fundamental principles of optical imaging
- operation of most widely used microscopy/interferometric techniques with particular regard to the nano-bio domain Expected skills are:
- ability to understand the underlying mechanisms of complex imaging systems
- ability to design and operate optical systems for specific imaging purposes

Classical Electromagnetism
Mathematical Analysis (Calculus).

Classical Electromagnetism
Mathematical Analysis (Calculus).

- Brief summary of classical electromagnetism and wave propagation
- Geometrical and physical Optics
- Mathematical description of image formation theory, Fourier optics
- Optical wide-field microscopy and scanning confocal microscopy
- Advanced fluorescence microscopy techniques
- Statistical Optics and speckles: spatial and temporal coherence of light
- Interferometric, holographic and adaptive image formation systems
- Control and manipulation of wave fronts: wavefront analysers, spatial light modulator devices
- Imaging through turbid media

- Brief summary of classical electromagnetism and wave propagation
- Geometrical and physical Optics
- Antenna theory examples
- Mathematical description of image formation theory, Fourier optics
- Optical wide-field microscopy and scanning confocal microscopy
- Advanced fluorescence microscopy techniques
- Optics in solids
- Aspects of theory of Cherence
- Interference and diffraction
- Control and manipulation of wave fronts: wavefront analysers, spatial light modulator devices
- Diffractive optics with examples to optical tweezers

The course involves class lectures, supported with electronic slides and old-fashioned blackboard writing; and class exercises.
During class exercises, exemplary applications will be considered, including calculations/simulations performed on laptop.
In addition, practical “hands-on” sessions will be organized, depending on the availability of research laboratories and scientific personnel.

The course involves class lectures and exercises, supported with electronic slides and board writing. During class exercises, exemplary applications will be considered.
Both lectures and exercises will be performed exclusively in class. In case of sanitary emergency conditions, online or blended modality will be considered.
In addition, practical “hands-on” sessions will be organized, depending on the availability of research laboratories and scientific personnel. Otherwise, the exp. demonstrations will be filmed and made available to students

J.W. Goodman, Introduction to Fourier Optics
J.W. Goodman, Statistical Optics
B.E.A. Saleh and M.C. Teich, Fundamental of Photonics
Additional supporting material provided during the course

Fowels, Introduction to Modern Optics
J.W. Goodman, Introduction to Fourier Optics
J.W. Goodman, Statistical Optics
B.E.A. Saleh and M.C. Teich, Fundamental of Photonics
Additional supporting material provided during the course

The exam includes a written and an oral discussion.
The written part consists of :
a) simple symbolic or numeric examples of the concepts explained in the course;
b) multiple-answer questions;
c) topical themes to be extensively discussed.
The total allotted time is 2 hrs. A total score of at least 18/30 is required to access to the oral examination, which will last 20-30 mins on average.

Gli studenti e le studentesse con disabilità o con Disturbi Specifici di Apprendimento (DSA), oltre alla segnalazione tramite procedura informatizzata, sono invitati a comunicare anche direttamente al/la docente titolare dell'insegnamento, con un preavviso non inferiore ad una settimana dall'avvio della sessione d'esame, gli strumenti compensativi concordati con l'Unità Special Needs, al fine di permettere al/la docente la declinazione più idonea in riferimento alla specifica tipologia di esame.

The exam includes a written and an oral discussion if required by the student.
The written part consists of :
a) exercice and symbolic or numeric examples of the concepts explained in the course;
b) multiple-answer questions;
c) 2 topical themes to be extensively discussed.
The total allotted time is 2 hrs. A total score of at least 18/30 is required to access to the oral examination, which will last 20-30 mins on average.

In addition to the message sent by the online system, students with disabilities or Specific Learning Disorders (SLD) are invited to directly inform the professor in charge of the course about the special arrangements for the exam that have been agreed with the Special Needs Unit. The professor has to be informed at least one week before the beginning of the examination session in order to provide students with the most suitable arrangements for each specific type of exam.

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Corso Duca degli Abruzzi, 24 - 10129 Torino, ITALY

Corso Duca degli Abruzzi, 24 - 10129 Torino, ITALY