Computational Modeling of the Interaction Between short peptide sequences (PAL) and CSA protein to develop optimized cancer chemotherapy drug delivery
Parole chiave COMPUTATIONAL MODELS, DRUG FORMULATION, PEPTIDES
Gruppi di ricerca 28- biomedica
Tipo tesi M.SC.
Descrizione Platelets are being developed as vehicles to deliver cancer chemotherapy drugs directly to cancer cells. The first task is to conjugate the recognition protein to the platelet anchoring sequence (PAL). To optimize the process we will be studying the interaction between PAL, an 11 amino acid long sequence and chondroitin sulfate A (CSA) whose crystal structure is know. CSA is the main GAG facilitating sequestration of proteins in alpha granules of platelets. To study the affinity of PAL for CSA, our collaborators based at the Sunnybrook Hospital in Toronto mainly use titration calorimetry, which has been able to detect a good interaction. It seems that the affinity of PAL to CSA is charge dependent, but the nature of the PAL/CSA interaction is unclear and we would like to study it with computational modeling, which is the objective of this project.
Conoscenze richieste molecular dynamics, bioinformatics, docking algorithms
Note This project will involve collaboration with researchers in Toronto, Canada who will provide experimental data and validation of computational predictions.
Scadenza validita proposta 21/10/2020 PROPONI LA TUA CANDIDATURA