Synthesis and characterization of polymeric nanoparticles to target muscular cells.
Tesi esterna in azienda
Riferimenti VALENTINA ALICE CAUDA
Riferimenti esterni Prof. Marta Guerra Rebollo
Prof. Cristina Fornaguera
Institut Quimico de Sarrža- Universidad Ramon Lull, Barcelona- Spain
Gruppi di ricerca AA - Materials and Processes for Micro and Nano Technologies
Tipo tesi SPERIMENTALE
Descrizione Skeletal muscle repair is facilitated by a population of dedicated muscle stem cells (MuSCs), also known as satellite cells, that reside in anatomically defined niches within muscle tissues. Promising therapies based on gene therapy aim to promote the production of DYSTROPHIN in muscular cells. However, these therapies displayed a major problem: dystrophic MuSCs exhibit defects leading to a strong reduction in the generation of myogenic progenitors needed for proper muscle regeneration. As a result, the efficiency of gene therapies is drastically diminished. In this scenario, to develop new safe and efficient therapies aimed at increasing the regeneration capabilities of MuSCs within dystrophic muscle is mandatory. Our previous results showed that microRNA miR-106b is an important modulator of MuSCs quiescence. MiR-106b down-regulation occurs during MuSCs activation and is required for proper muscle repair in vivo. Interestingly, miR-106b is increased in MuSCs of dystrophic mice, and intramuscular injection of anti-mir-106b enhances their skeletal muscle regenerative potential. In this project, we propose to deliver miRNA-sponges specifically directed at blocking miR-106b in MuSCs by using polymeric nanoparticles in order to boost muscle regeneration in DMD context.
Conoscenze richieste nanoparticelle, caratterizazzione di nanoparticelle, basi di biologia e chimica, scienza dei materiali
Note Methodologies: Synthesis of the muscular targeted OM-PBAE polymers, coating of miRNA sponges, nanoparticle characterization, cell culture of muscular and non-muscular cells, transduction experiments, cell cytometry, confocal microscopy in vivo biodistribution assays.
Scadenza validita proposta 15/12/2023 PROPONI LA TUA CANDIDATURA