KEYWORD |
Experimental investigations of the effects of electromagnetic fields on the activity of neuronal cultures
Parole chiave ACTION POTENTIAL, HYPERPOLARIZED LIGHT, INFRARED LIGHT, METABOLIC ACTIVITY, NEURONAL CULTURE, PHOTOBIOMODULATION
Riferimenti JACEK ADAM TUSZYNSKI
Gruppi di ricerca 28- biomedica
Tipo tesi EXPERIMENTAL
Descrizione This research will be aimed at investigating the effects of two distinct light exposure systems, the Bioptron device (hyperpolarized light) and the Vielight NeuroPro device (InfraRed ra- diation), on cellular responses in neuronal cultures The study will focus on elucidating the impact of different irradiation conditions on cell viability, action potential propagation, ATP production, and metabolic shifts. Cells (neurons and possibly glial cells) will be cultured in standard conditions and exposed to light using the Bioptron device (40 mW/cm2) or the Vielight NeuroPro device (60 mW/cm2, 10 Hz frequency sweep). Exposure times will range from 10 to 60 minutes, with some groups receiving intermittent breaks. Cell viability will be assessed using the Alamar blue assay, while morphological changes will be evaluated through immunofluorescence staining of actin, tubulin, and mitochondria. ATP production and metabolic shifts were analyzed using a Glycolysis/OXPHOS Assay kit. Using patch clamp technology we will investigate the changes in the action potential propagation.
Conoscenze richieste basic cell culture techniques
most of the other methods will be learned during the research project's duration
Note Most of thew work will be performed at the University of Alberta, Edmonton, Canada
Scadenza validita proposta 06/01/2026
PROPONI LA TUA CANDIDATURA